Journal: Immunologic research
Article Title: RGC-32 regulates reactive astrocytosis and extracellular matrix deposition in experimental autoimmune encephalomyelitis
doi: 10.1007/s12026-018-9011-x
Figure Lengend Snippet: Localization of ECM components in MS plaque. Collagen I (a), collagen II (b), collagen III (c), collagen IV (d), collagen V (e), and fibronectin (f) were localized by immunostaining in MS plaques, around the blood vessels and in the parenchyma. Collagens I, III, IV, and V form a meshwork in the perivascular space in MS plaques. In active lesions, collagen V is found around blood vessels and in the perivascular space as well as in parenchymal areas outside the PVS (e). In the normal brain, collagen I (g) and collagen III (h) were detected at significantly lower levels than in MS plaques. Control for the immunoperoxidase reaction was negative (i). Nova RED was used as a substrate for immunostaining and hematoxylin for counter staining. Original magnifications: (a)–(d) and (f)–(i): × 200, (e): × 400
Article Snippet: The slides were washed several times in PBS and reacted with alkaline phosphatase-conjugated goat anti-mouse (Sigma-Aldrich), diluted 1/400 and processed with Vector alkaline phosphatase substrate kit III (Vector Labs) or Nova RED as the chromogen substrate.
Techniques: Immunostaining, Staining